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rat vascular smooth muscle cells (smcs; lonza, r-asm-580)  (Lonza)


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    Structured Review

    Lonza rat vascular smooth muscle cells (smcs; lonza, r-asm-580)
    Rat Vascular Smooth Muscle Cells (Smcs; Lonza, R Asm 580), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+vascular+smcs/human+vascular+smooth+muscle+cells/pm35399536-29-0-6
    Average 90 stars, based on 1 article reviews
    rat vascular smooth muscle cells (smcs; lonza, r-asm-580) - by Bioz Stars, 2026-09
    90/100 stars

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    Isolation:

    Article Title: Matrix mechanotransduction mediated by thrombospondin-1/integrin/YAP in the vascular remodeling
    Article Snippet: The RNA-seq data were deposited in National Center for Biotechnology Information's (NCBI’s) Gene Expression Omnibus (GEO) database ( https://www.ncbi.nlm.nih.gov/geo/ ); the GEO accession number is {"type":"entrez-geo","attrs":{"text":"GSE131750","term_id":"131750"}} GSE131750 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= {"type":"entrez-geo","attrs":{"text":"GSE131750","term_id":"131750"}} GSE131750 ). .. Rat vascular SMCs (Lonza, R-ASM-580, isolated from the aorta of 150 to 200 g adult male Sprague-Dawley rats) were grown in Dulbecco's modified Eagle medium (DMEM) with 20% (vol/vol) fetal bovine serum (FBS) and 1× Antibiotic-Antimycotic (ThermoFisher Scientific). .. HUVECs were grown in HuMedia-MvG (KURABO) supplemented with 5% (vol/vol) FBS and growth factors.

    Article Title: Role of PAR1-Egr1 in the Initiation of Thoracic Aortic Aneurysm in Fbln4-Deficient Mice
    Article Snippet: .. Rat vascular SMCs (Lonza, R-ASM-580, isolated from the aorta of adult male Sprague-Dawley rats) were cultured in DMEM/F12 media (Thermo Fisher Scientific, #11320033) with 20% FBS and 1% of Antibiotic-Antimycotic. .. Rat vascular SMCs were cultured with DMEM/F12-serum-free media for 24 hours, then re-cultured in DMEM/F12-serum-free media with 25 Unit/mL of thrombin from bovine plasma (SigmaAldrich, #9002044) for indicated time points (0, 0.5, 1, 3, and 6 hours).

    Modification:

    Article Title: Matrix mechanotransduction mediated by thrombospondin-1/integrin/YAP in the vascular remodeling
    Article Snippet: The RNA-seq data were deposited in National Center for Biotechnology Information's (NCBI’s) Gene Expression Omnibus (GEO) database ( https://www.ncbi.nlm.nih.gov/geo/ ); the GEO accession number is {"type":"entrez-geo","attrs":{"text":"GSE131750","term_id":"131750"}} GSE131750 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc= {"type":"entrez-geo","attrs":{"text":"GSE131750","term_id":"131750"}} GSE131750 ). .. Rat vascular SMCs (Lonza, R-ASM-580, isolated from the aorta of 150 to 200 g adult male Sprague-Dawley rats) were grown in Dulbecco's modified Eagle medium (DMEM) with 20% (vol/vol) fetal bovine serum (FBS) and 1× Antibiotic-Antimycotic (ThermoFisher Scientific). .. HUVECs were grown in HuMedia-MvG (KURABO) supplemented with 5% (vol/vol) FBS and growth factors.

    Patch Clamp:

    Article Title: Engineered Endothelial Progenitor Cells That Overexpress Prostacyclin Protect Vascular Cells
    Article Snippet: .. Whole-cell patch-clamp studies Native EPCs or PGI2-EPCs (5×10 4 ) were cocultured for 12 hours with rat vascular SMCs (rSMCs, Lonza) under normal (20% O 2 , 5% CO 2 , 37°C) or hypoxic (1.1% O 2 , 5% CO 2 , 37°C) conditions in a double-chamber transwell culture system (BD Falcon, Rockville, MD). .. SMCs were harvested, and the single-cell K+ current (I K ) was measured using the whole-cell patch-clamp technique with an Axopatch 200B amplifier and a CV-4 headstage (Axon Instruments Inc., Union City, CA).

    Cell Culture:

    Article Title: Abnormal mechanosensing and cofilin activation promotes the progression of ascending aortic aneurysms in mice
    Article Snippet: .. Cell Culture, Transfection and Immunostaining Rat vascular SMCs (Lonza) were maintained in DMEM/F12 supplemented with 20% fetal bovine serum and 0.1% GA-1000, containing 30 μg/ml Gentamicin and 15 ng/ml Amphotericin (Lonza). .. For immunostaining, cells were grown on 4-well chamber slide (Thermofisher) and the plasmid DNA encoding human Slingshot-1L (WT or CS, a phosphatase mutant) fused with YFP (generous gift from Dr. Kensaku Mizuno), were transfected into cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Role of PAR1-Egr1 in the Initiation of Thoracic Aortic Aneurysm in Fbln4-Deficient Mice
    Article Snippet: .. Rat vascular SMCs (Lonza, R-ASM-580, isolated from the aorta of adult male Sprague-Dawley rats) were cultured in DMEM/F12 media (Thermo Fisher Scientific, #11320033) with 20% FBS and 1% of Antibiotic-Antimycotic. .. Rat vascular SMCs were cultured with DMEM/F12-serum-free media for 24 hours, then re-cultured in DMEM/F12-serum-free media with 25 Unit/mL of thrombin from bovine plasma (SigmaAldrich, #9002044) for indicated time points (0, 0.5, 1, 3, and 6 hours).

    Transfection:

    Article Title: Abnormal mechanosensing and cofilin activation promotes the progression of ascending aortic aneurysms in mice
    Article Snippet: .. Cell Culture, Transfection and Immunostaining Rat vascular SMCs (Lonza) were maintained in DMEM/F12 supplemented with 20% fetal bovine serum and 0.1% GA-1000, containing 30 μg/ml Gentamicin and 15 ng/ml Amphotericin (Lonza). .. For immunostaining, cells were grown on 4-well chamber slide (Thermofisher) and the plasmid DNA encoding human Slingshot-1L (WT or CS, a phosphatase mutant) fused with YFP (generous gift from Dr. Kensaku Mizuno), were transfected into cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions.

    Immunostaining:

    Article Title: Abnormal mechanosensing and cofilin activation promotes the progression of ascending aortic aneurysms in mice
    Article Snippet: .. Cell Culture, Transfection and Immunostaining Rat vascular SMCs (Lonza) were maintained in DMEM/F12 supplemented with 20% fetal bovine serum and 0.1% GA-1000, containing 30 μg/ml Gentamicin and 15 ng/ml Amphotericin (Lonza). .. For immunostaining, cells were grown on 4-well chamber slide (Thermofisher) and the plasmid DNA encoding human Slingshot-1L (WT or CS, a phosphatase mutant) fused with YFP (generous gift from Dr. Kensaku Mizuno), were transfected into cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions.



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